- Dry Bones: A drug-addicted Koopa Troopa and the main protagonist.
- Boo: A ghost who is the second main protagonist. He assists Dry Bones in making things scary.
- Dry Bowser: Dry Bones's master and the leader of the Dry Bones. He oversees his progress.
- King Boo: Boo's father and the king of the Boos. He oversees and provides funding for Boo's adventure.
- King Sombra: An evil pony and the main antagonist. He tries to prevent Dry Bones and Boo from spooking things up.
- Shy Guy: An awesome dude who wears a mask. He is good friends with Dry Bones.
- Metal Mario: A drug-addicted Italian plumber who is also one of Dry Bones's drug buddies.
- SKODWARDE: A sinister octopus who tries to stop Dry Bones and Boo's journey.
- Elmo: The furry red faggot from Sesame Street who always dies.
See List of episodes of Dry Bones's and Boo's Haunting Hour for more information.